Single-cell sequencing and second-generation RNA sequencing-based screening of macrophage-associated marker genes for recurrent miscarriage diagnosis

We profiled the transcriptomes of about 110000 single cells from 16 human first-trimester decidual and villi samples from 5 normal samples and 3 recurrent miscarriage (RM) samples. Each decidual and villi samples was collected from the same patient. The cellular composition revealed five major subsets of immune cells including NK, T cell, macrophage, monocytes and B cell in the maternal fetal interface. We used the marker gene of macrophages to establish the diagnosis and treatment model of unexplained recurrent abortion, in order to explore the relationship between immune cells and unexplained recurrent abortion. Profiling the transcriptomes of about 110000 single cells from 16 human first-trimester decidual and villi samples from 5 normal samples and 3 recurrent miscarriage (RM) samples. Each decidual and villi samples was collected from the same patient using single-cell RNA Seq. Decidua and villi samples with the same number (such as NC_D6-1 and NC_V6-1, RM_D6 and RM_V6) were collected from the same patient.