Segregation analysis of ala3 indicates a temperature-sensitive defect in transmission through the male gametophyte.

Under unstressed conditions, the observed results were compared to an expected Mendelian segregation. Results of assays performed under hot-day/cold-night temperature stress conditions (Figure S2) were compared to the results of the same assay performed under unstressed conditions. Statistical significance was determined by the Pearson’s Chi-Squared test.aExpected percentages based on Mendelian segregation.bExpected percentages based on unstressed results.