Construction and characterization of coronavirus nonstructural protein 3-host protein interaction networks unravel an important role of cleavage and polyadenylation specificity factor 6 in regulation of viral RNA replication

To investigate the host proteins that interact with nsp3 in coronavirus-infected cells and their functional roles, we conducted an affinity mass spectrometry on nsp3-expressing cells to analyze the potential interactions between host proteins and nsp3 from IBV and SARS-CoV-2