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H3K27ac ChIP-seq for primary multiple myeloma patients' samples (MM1-MM10)

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https://www.ncbi.nlm.nih.gov/sra/SRP250674
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ChIP-seq analysis was performed in primary Multiple Myeloma patients' samples to analyze acetylation of histone H3K27ac. Overall design: Primary multiple myeloma patients' samples (MM1-MM10) were crosslinked with formaldehyde for 10 min. DNA was enriched by chromatin-immunoprecipitation (ChIP) and analyzed by Illumina Hiseq 4000. A sample of whole cell extract (WCE) was sequenced and used as the background to determine enrichment. ChIP was performed using an antibody against histone H3 lysine 27 acetylation (H3K27Ac: Abcam, ab4729).
创建时间:
2021-02-18
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