TABLE 1 in Microfungi associated with dead caterpillars and spiders in Xishuangbanna Tropical Botanical Garden, P. R. China
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TABLE 1. The PCR conditions and primers used in this study. LocusDefinitionPrimerPrimer DNA sequence (5'-3')Annealing (PCR)Referencesgapdhglyceraldehyde 3-phosphate dehydrogenasegpd1CAACGGCTTCGGTCGCATTGgpd2GCCAAGCAGTTGGTTGTGCa60 °C, 30 s bBerbee et al. (1999)ITSinternal transcribed spacerITS4TCCGTAGGTGAACCTGCGGITS5TCCTCCGCTTATTGATATGCa55 °C, 50 sbWhite et al. (1990)LSUnuclear ribosomal large subunitLR0RGTACCCGCTGAACTTAAGCLR5ATCCTGAGGGAAACTTCa55 °C, 50 sbVilgalys & Hester (1990)rpb1DNA-dependent RNA polymerase II largest subunitAfGARTGYCCDGGD CAYTTYGGCrCGCATTCTTTCCATTAa49 °C, 1 minbLu et al. (2013)SSU18S ribosomal RNANS1GTAGTCATATGCTTGTCTCNS4CTTCCGTCAATTCCTTTAAGa55 °C, 50 sbWhite et al. (1990)tef1-αtranslation elongation factor 1 alpha983FGCYCCYGGHCAYCGTGAYTTYAT2218RATGACACCRACRGCRACRGTYTGa56 °C, 50 s bRehner & Buckley (2005)
