MultiBarcodeTools: Supplementary Data

Supplementary Data of MultiBarcodeTools. refDB_34_Primers_fish.tsv.gz: a reference database for amplicons of 34 fish eDNA metabarcoding primers predicted using CRABS v0.1.3 (Jeunen et al., 2022) by targeting 3,482 fish species with complete mitogenomes. Minamoto_Simulate.tar.gz: taxonomy identification results from the standalone MiFish Pipeline on real and simulated data from samples from the Sagami River, Japan (Takasaki et al., 2021). Teleo_and_Riaz.tar.gz: taxonomy identification results from the standalone MiFish Pipeline on sequencing data from samples from the pond in Linkebeek, Belgium (Brys et al., 2021). refDB_19_Primers_insect.tsv.gz: a reference database for amplicons of 19 insect eDNA metabarcoding primers predicted using CRABS v0.1.3 (Jeunen et al., 2022) by targeting 4,484 insect species with complete mitogenomes. Note: three primers have no positive hits and are not included in the file. refDB_3_Primers_fungi.tsv.gz: a reference database for amplicons of three fungal eDNA metabarcoding primers predicted using CRABS v0.1.3 (Jeunen et al., 2022) by targeting 14,615 fungal species with ITS reference sequences. matrix.MultiBarcodePipeline.Result.Insect.xlsx: results produced by MultiBarcodePipeline using the command: multi-barcode refDB_19_Primers_insect.tsv -p cox1.mICOllintF_jgHC02198 matrix.MultiBarcodePipeline.Result.Fungi.xlsx: results produced by MultiBarcodePipeline using the command: multi-barcode refDB_3_Primers_fungi.tsv msa.example.tar.gz: Full length sequences mentioned in Figure S5-S7