The Bromodomain and extraterminal (BET) inhibitor attenuates inflammatory response and cell migration in human microglia. The Bromodomain and extraterminal (BET) inhibitor attenuates inflammatory response and cell migration in human microglia

We investigated the mechanisms underlying the anti-inflammatory and anti-migratory function and genes targeted by JQ1 in lipopolysaccharide (LPS)-activated human microglia HMC3 cells using RNA sequencing. The BET inhibitor JQ1 attenuates the LPS-induced inflammatory and migratory response. In addition, LPS-induced IRF1 was inhibited by JQ1, and IRF1 suppressed microglial migration by regulating inflammation and migration genes. Overall design: The gene expression profile was performed with control HMC3 (Con), JQ1-treated HMC3 (JQ1), LPS-treated HMC3 (LPS), LPS+JQ1-treated HMC3 (LPS+JQ1). Samples were generated by deep sequencing, in triplicate, using Illumina 2500.