Mutations generated by exonuclease-deficient Pol ε <i>in vitro</i>.

The results for the holoenzyme are from [40]. For both enzymes, only phenotypically detectable changes in the lacZ gene are included. aOther mutations include deletions of 2–3 bases, more complex substitution-deletions, and deletions of larger numbers of bases between direct repeat sequences. Statistical analysis of the distributions of substitutions produced by the four subunit and two-subunit pol ε along lacZ was performed using the COLLAPSE program [70].These two spectra are not different (P = 0.90). This result strongly suggest that properties of four subunit and two subunit polymerases are highly similar (linear correlation coefficient for the two spectra = 0.72, P<0.01). We also compared the raw data from Table 2 using the same approach. These two distributions are different (P = 0.005). However, they are not different after the removal of the category “Other mutations” (P = 0.11). The only reason why two spectra are different are long deletions (>100 bp) that are included under “Other mutations.” After removal of these long deletions the spectra are not different (P = 0.17).