Genome-wide expression profiling of Octopus Arm and Hatchlings

This study was carried out to investigate the gene expression profile from arm and hatchlings transcriptome across multiple individual Octopus bimaculoides . Overall design: 3 different arm sections from an adult male Octopus bimaculoides were collected (containing all tissues, including skin, sucker, muscle and nerve). 2 arm tips were collected from a different adult male and one adult female. One whole-body 30 day old hatchling was removed from chorion and anesthetized. All animals were anesthetized in ethanol, all samples were dissected in sea water and flash frozen. RNA was extracted using Trizol and DNAse treated to carry out Ribo-Zero library preparation.