TuxNet: A simple interface to process RNA sequencing data and infer gene regulatory networks [XVE:PAN]

We obtained transcriptional profiles of roots from an XVE:PAN transgenic line under the control of the ß-estradiol-inducible promoter, at times 0, 6, and 24 hours after induction of PAN expression. Overall design: XVE:PAN seeds were first grown on 1X MS supplemented with 1% sucrose plates, and then were transferred to 1X MS supplemented with 1% sucrose and 10 uM ß-estradiol for 0, 6, or 24 hour PAN induction. RNA was then exctracted from the roots. All XVE:PAN seeds were grown for a total of 5 days.