Depot-dependent transcriptomic profile of human adipose tissue macrophages

Macrophages from visceral and subcutaneous white adipose tissue (vWAT and sWAT, respectively) can have opposing roles in the systemic metabolic changes associated with obesity. We evaluated the gene expression in CD11b positive macrophage cells from vWAT and sWAT and in the whole adipose depots. A Clariom D-based transcriptomics analysis was performed in macrophages magnetically separated from vWAT and sWAT from patients with obesity (Cohort 1; N = 11). The experimental design for hybridization processing included 4 batches of 9-11 samples each with balancing among different batches between the two compared conditions: vWAT and sWAT.