ReNcell differentiation: Phosphoproteomics during differentiation under basal conditions. Dataset 2 of 3

ReNcell-VM neuroprogenitors were differentiated by growth factor (EGF and FGF) removal for 15 days in culture, giving rise to dopaminergic neurons, astrocytes, and oligodendrocytes. Cells were collected at 10 time points and processed for phosphoproteomics by liquid chromatography mass spectrometry (LC/MS) using 10-plex tandem mass tag (TMT) labelling combined with phosphopeptide enrichment by titanium dioxide (TiO2), allowing deep quantification of proteome dynamics across ~3,500 phospho-proteins (~11,000 sites of phosphorylation).