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Control of FoxO1 mRNA expression by microRNAs

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NIAID Data Ecosystem2026-03-13 收录
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE130181
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To identify miRNAs, a microarray analysis were differentially expressed between C2C12 myotubes stimulated with 1 μM clenbuterol for 1 h and their control myotubes. RNA was isolated using the mirVana™ miRNA Isolation Kit (Ambion, Austin, TX, USA) in accordance with the manufacturer’s instructions. All samples were diluted to a final concentration of 3 ng/mL. miRNA was hybridized to Affymetrix GeneChip microRNA 4.0 arrays (Affymetrix, CA, USA). miRNA array processing was performed according to the manufacturer's protocol.
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2022-03-09
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