Comparison between opioid (morphine) and vehicle treatments in mouse trigeminal ganglia and nucleus accumbens

Chronic opioid treatment elicits hyperalgesia in mice that is significantly augmented by a human migraine trigger and reduced with migraine therapy. The purpose of this study was to profile mRNA levels (transcriptome) to understand the differences between opioid (morphine) and vehicle treatments in two nervous system regions, the trigeminal ganglia and the nucleus accumbens. Mice received 10?mL/kg intraperitoneal injections of either morphine or vehicle volume twice daily (s.c.) for 4 days where on days 1-3 the dose of morphine was 20 mg/kg, and on day 4 it was 40 mg/kg. Overall design: RNA samples were obtained from 10 mice 24 hours after the last injection and were subjected to paired-end RNA sequencing