Selection of genes illustrating various patterns of SNPs (dN and dS) distribution across the 18 isolates indicating diverse evolutionary forces acting on ORF across L. tropica populations.

*The comparison of the mean dN+dS between population II (the reference genome of isolate L590 is more closely related to these isolates, S1 Fig) and the population I, III and IV (all with three or more insolates) highlight genes with distinct trends between these populations. This suggests differential evolutionary forces and strength act on these genes across these populations. The genes are ranked according to the dN/dS ratio for genes from LT2. The bottom four genes illustrate ORF experiencing purifying selection among the analysed L. tropica isolates and include two genes that have, in contrast, evidence for positive selection in L. donovani [21].