FeCγ25 mice show a decreased number of immature neurons in the SGZ of dentate gyrus (DG).

A–B, Coronal sections from 3 mo (A) and 12 mo (B) brains of wild-type and FeCγ25 mice were immunostained for the immature neuronal marker doublecortin (DCX). There is an abundance of immature neurons in the SGZ of the granule cell layer in both the supra- and infra-pyramidal blades of the DG. Compared to the wild-type (top) there is a significant decrease in the number of DCX+ cells in the SGZ of FeCγ25 mice (bottom). C, Magnification of the boxed region in (A) show the diminished number of DCX+ cells in FeCγ25 mice at 3 mo of age with a concomitant decline in dendritic number. (D) Quantitative analysis of the total number of DCX+ cells throughout the entire rostro-caudal extent of the hippocampus in 3-month-old (left) and 12-month-old (right) animals reveal a statistically significant decrease in the number of DCX+ cells in the SGZ of FeCγ25 mice compared to wild-type mice. Scale bar  = 100 µm. n = 4 for all.