Sequential appetite suppression by oral and visceral feedback to the brainstem

The termination of a meal is controlled by dedicated neural circuits in the caudal brainstem. A key challenge is to understand how these circuits transform the sensory signals generated during feeding into dynamic control of behaviour. The caudal nucleus of the solitary tract (cNTS) is the first site in the brain where many meal-related signals are sensed and integrated, but how the cNTS processes ingestive feedback during behaviour is unknown. Here, we describe how prolactin-releasing hormone (PRLH) and GCG neurons, two principal cNTS cell types that promote non-aversive satiety, are regulated during ingestion. PRLH neurons showed sustained activation by visceral feedback when nutrients were infused into the stomach, but these sustained responses were substantially reduced during oral consumption. Instead, PRLH neurons shifted to a phasic activity pattern that was time-locked to ingestion and linked to the taste of food. Optogenetic manipulations revealed that PRLH neurons co..., , # Data from: Sequential appetite suppression by oral and visceral feedback to the brainstem

This readme file provides instructions on reproducing the results presented in the research article titled \"Sequential appetite suppression by oral and visceral feedback to the brainstem,\" published by Ly et al. in Nature, 2023. The article can be accessed at [https://www.nature.com/articles/s41586-023-06758-2](https://www.nature.com/articles/s41586-023-06758-2). We have submitted our raw data for main figure 1 (**Figure_1_Data.xlsx**), main figure 2 (**Figure_2_Data.xlsx**), main figure 3 (**Figure_3_Data.xlsx**), main figure 4 (**Figure_4_Data.xlsx**), main figure 5 (**Figure_5_Data.xlsx**), and main figure 6 (**Figure_6_Data.xlsx**).

## Descriptions

### Figure 1 Data

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* Figure 1b
* Mean z-score 0-30 min: Average z-scored change in calcium activity during intragastric infusions for individual animals
* Figure 1c
* Mean z-score 0-10 min: Average z-scored change in calcium activity du...,