Assessment of Endocrine Disruption in the Shenandoah River Watershed – Transcriptome Data from Mobile Laboratory Fish Exposures and Other Experiments Conducted during 2014

Here, we employed integrated chemical and biological analyses to determine how environmental mixtures affected biological responses in watersheds with different landuse. Adult male fathead minnows (Pimephales promelas) were exposed to water from different locations within the Shenandoah River watershed (VA, USA) in 2014, 2015, and 2016. The exposure locations were chosen to capture unique landuse in surrounding watersheds, including agricultural, municipal, mixed-use, and forested sites. Gene expression profiles were measured in livers of male fish exposed for 7 days using Agilent 60K custom FHM microarrays. Independent statistical analyses were conducted for each year, using a reference site (PCFH) as the comparison. Differentially expressed probes were identified by one-way ANOVA followed by FDR correction. This study identified site-specific patterns of differentially expressed genes. ---------------------------------- NFSRD - North Fork Shenandoah River above tributary near Woodstock, VA NRWD - North River below WWTP near Mount Crawford, VA NRWU - North River above WWTP near Mount Crawford, VA PCFH - Passage Creek at fish hatchery at Waterlick, VA SFSR - South Fork Shenandoah River above WWTP at Bentonville, VA