Polycomb regulates sleep homeostasis in a specific group of cells in Drosophila brain

Sleep is regulated by the circadian rhythm and homeostasis. Previous studies have shown that epigenetic factors play a crucial role in controlling sleep homeostasis. However, a global view of the epigenetic target genes involved in sleep homeostasis is still lacking. In this study, we have discovered that Polycomb (Pc) regulates sleep in EB1-Gal4 and R69F08-Gal4 co-expressing cells, which are important for controlling sleep homeostasis. Using Targeted DAM-ID (TaDa) and RNAseq, we have identified Pc targets in EB1-Gal4 and R69F08-Gal4 expressing cells and analyzed Pc targets that are crucial for the control of sleep homeostasis. Furthermore, identifying common targets in EB1-Gal4 and R69F08-Gal4 marked cells provides a global view of the cluster-specific target genes of Pc, which potentially play a crucial role in controlling sleep homeostasis. The clustering of these target gene profiles ..., Fly strains and rearing
All fly stocks were reared on standard corn meal/agar fly food at 25°C, humidity of 60% and light dark cycle of 12:12 LD (12 hours of light followed by 12 hours of darkness). The following fly lines were used in this study: w1118 (BDSC: 5905), EB1-Gal4 (BDSC: 44409), R69F08-Gal4 (BDSC: 39499), UAS-RedStinger (BDSC: 8547) are from the Bloomington stock center (BDSC). UAS-PcRNAi-1 (THU1990), UAS-PcRNAi-2 (THU1306), UAS-GABA-B-R1RNAi-1 (THU2990), UAS-GABA-B-R1RNAi-2 (THO1472.N), UAS-GrdRNAi (THU5922), UAS-SecClRNAi (THU2480), UAS-CG12344RNAi (THU2249), UAS-nAChRalpha3RNAi (THU2756), UAS-HdcRNAi (THU2140), UAS-HisCl1RNAi (THU2851), UAS-Daao1RNAi (THO4274.N), UAS-Daao2RNAi (THO4297.N) are from the TsingHua Fly Center (THFC). UAS-stop>kir2.1; LexAop-Flp, UAS-stop>TNT; 8×LexAop-Flp, LexAop-Flp; UAS-stop>shits, 8×LexAop-Flp; UAS-stop>TrpA1, LexAop-kir2.1, UAS-S..., # Polycomb regulates sleep homeostasis in a specific group of cells in Drosophila brain

Dataset DOI: [10.5061/dryad.nzs7h4545](10.5061/dryad.nzs7h4545)

## Description of the data and file structure

This dataset was generated to test whether Polycomb (Pc) regulates sleep amount and sleep homeostasis in *Drosophila* and to identify the cell types and downstream genes involved. We combined cell-type–specific genetics (EB1-Gal4, R69F08-Gal4, Repo-Gal4; UAS-Pc RNAi; Kir2.1/TNT; dTrpA1; LexA/LexAop intersections) with behavioral recording under LD cycles. Sleep was measured as 24-h profiles, total sleep, and recovery after 12-h night sleep deprivation. Brain expression patterns and co-localization were assessed by immunostaining. Pc targets were mapped by TaDa (Targeted DamID) in the above populations and by RNA-seq after Pc perturbation, followed by GO/KEGG enrichment. The files provide the raw/processed data underlying the main and supplementary figures/tables.

### Files and variables

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