Field-realistic exposure to the novel insecticide flupyradifurone reduces reproductive output in a bumblebee (Bombus impatiens)

Novel insecticides are continuously being developed for application in response to increased legal restriction of previously developed insecticides and resistance in target insects. These novel insecticides, such as flupyradifurone (FPF), remain relatively untested on non-target organisms, including bumblebees. Further, existing tests on honeybees tend to focus on adult mortality, and thus sub-lethal effects, such as impacts on reproductive output, are neglected, despite their importance for population-level impacts. To address if the novel insecticide FPF has sub-lethal effects on bumblebee reproduction and behavior, we established microcolonies and chronically exposed them to field-realistic concentrations over a 14-day period. We found that exposure to FPF reduced the bumblebees’ reproductive output in terms of the number of larvae produced and the mean mass of each larval instar. FPF-treated bees also stored less sucrose and constructed fewer honeypots. However, adult bumblebee mort..., , , # Field-realistic exposure to the novel insecticide flupyradifurone reduces reproductive output in a bumblebee (Bombus impatiens)

[https://doi.org/10.5061/dryad.r4xgxd2nk](https://doi.org/10.5061/dryad.r4xgxd2nk)

The accompanying three excel files contain all of the data from the above titled manuscript. Description below explains what information is within each file. 

## Description of the data and file structure

FPF_microcolony_data_archive.xlsx has most of the main data. The first sheet contains general information about each microcolony. This includes the date that the experiment began, the queenright colony that the bees were taken from (indicated by a letter in the column titled \"natal\"), the microcolony ID number, the treatment group (\"C\" = control, \"L\" = low, \"M\" = medium, \"H\" = high, and \"P\" = positive control). This first sheet also contains the information about the total volume of sucrose stored within each microcolony at the end of the 14 day experiment, as well as the ...