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APPLICATION OF GhPRXR1 PROTEIN AND CODING GENE THEREOF IN REGULATING AND CONTROLLING OIL CONTENT OF COTTONSEED

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国家林业和草原科学数据中心2023-02-12 更新2024-03-07 收录
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The invention discloses application of GhPRXR1 protein and coding gene thereof in regulating and controlling oil content of cottonseed. The invention clones cotton GhPRXR1 gene from upland cotton material Msco-12, successfully constructing yeast over-expression vector and transforming saccharomyces cerevisiae. Compared with the trans-empty vector yeast control, the oil content of the 3 positive transgenic yeasts is respectively improved by 20.01%, 34.79% and 37.25%. The invention also successfully constructs a virus-induced GhPRXR1 gene silencing vector and transforms upland cotton Msco-12. The average oil content of 6 GhPRXR1 gene silencing cotton positive individual mature cottonseed is reduced by 18.11% compared with that of 3 control individual mature cottonseeds, indicating that the GhPRXR1 gene can regulate and control oil content of cottonseed, having great value in improving the cottonseed yield and cotton breeding.
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国家林业和草原科学数据中心
创建时间:
2023-02-12
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