A cytochrome P450 CYP87A4 imparts sterol side-chain cleavage in digoxin biosynthesis

Identifying the foxglove P450scc through differential transcriptomic analysis. Leaf and root tissues from three different seedlings were used to prepare the Illumina sequence library in duplicate. Total RNA was isolated using the RNeasy Plant Mini Kit (Qiagen, Germantown, MD, USA). The sequencing library was prepared from total RNA using the TrueSeq Ribo-Zero Plant RNA library prep kit (Illumina, San Diego, CA, USA) that removes ribosomal RNA. A quality check of the library was carried out with an Agilent 2100 bioanalyzer. The library was sequenced using Illumina HiSeq 2500 to generate 100 bp paired-end raw reads.