Transcript profiling in peritoneal macrophages from wildtype, Fra-1ΔMxCre or Fra-2ΔLysMCre mice

The polarization and activation of macrophages are controlled synergistically by transcription factors such as NF-κB and AP-1 transcription factor members. Surprisingly, little is known about the role of the Fra proteins, both members of the AP-1 transcription factor family, in macrophage activity. To determine the full profile of Fra network, microarray RNA expression analysis using Agilent Technologies platform was performed in wild-type, Fra-1ΔMxCre or Fra-2ΔLysMCre macrophages. For microarray analyses, peritoneal macrophages were collected by peritoneal lavage of 8 week old wild-type (n=4), Fra-1ΔMxCre (n=2) or Fra-2ΔLysMCre (n=2) mice. Peritoneal cells were plated over night. The next day, suspension cells were removed and only adherent cells were used for RNA isolation.