Strain will be used for comparative genome analysis. Mycoplasma mycoides subsp. mycoides SC str. Gladysdale

Mycoplasma mycoides subsp. mycoides SC. This subspecies is the etiologic agent of CBPP. The Gladysdale strain was isolated prior to 1964 from cattle in Australia and has been widely used as a reference strain for several studies (Gaurivaud et al., 2004). The source used for this project was a stock maintained at the Foreign Animal Disease Diagnostic Laboratory, USDA, APHIS, at the Plum Island Animal Disease Center, which was routinely used to induce and demonstrate acute pulmonary disease in cattle following tracheal inoculation. Consequently this represents an experimentally pathogenic strain. To minimize mutational heterogeneity in the genome sample, DNA was prepared from an axenic culture propagated from a single colony isolate from this stock (MU clone SC5; available from European repository under proper regulatory controls). The genome, a single circular chromosome, was sequenced to closure using the Sanger whole genome random shotgun method, yielding approximately 8-fold sequence coverage. Generally the sequence resembles that of type strain PG1, derived from non-pathogenic culture (Manso-Silván et al., 2009), but displays many differences ranging from single nucleotide substitutions to duplications or transpositions involving larger repeats, both known and previously unknown. This genome sequence and the clonal isolate it represents provide an opportunity to examine pathogenesis and population dynamics on an experimental and genomic level. This project was supported by US Department of Agriculture ARS Project 1940-32000-039-08S at the University of Missouri and the Office of Science (BER), U.S. Department of Energy, Grant No. DE-FC02-02ER63453 to the J. Craig Venter Institute.