Overexpression of a-SMA led to differential gene expression in INS-1 cells (RNA-seq)

The goal of this study was to define the cohort of genes whose expression is regulated by a-SMA function in type 2 diabetic mellitus . Towards this goal, INS-1 cells stably expressing a-SMA (INS-SMA) were established. In addition, a control cells (INS-MOCK) was generated by transfection with empty vector. Subsequently, total RNA was isolated and then performed transcriptome RNA sequence. Overall design: We had two groups: (1) INS-MOCK and (2) INS-SMA. Each group had 3 biological replicates.