The effect of acidosis on adipose-derived stem cell

The acidic microenvironment plays a key role to result in the poor survival and limited function of transplanted stem cells in ischemic diseases.In this study, different pH levels of medium were performed to treat adipose-derived stem cells (ADSCs) with 24/48h time- exposure, to explore the effect of extracellular acidosis on ADSCs. Adipose-derived stem cells (ADSCs) were treated with different pH levels of medium( pH=7.4/7.2/7.0/6.5) for 24h/48h, to explore the transcriptic alteration following with extracellular acidosis.