Ribosomal profiling of adipocytes
收藏Alliance of Genome Resources2026-08-01 收录
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Purpose: To generate gene expression profiles of inguinal white, epididymal white and interscapular brown adipocytes Methods: Translating ribosomal affinity purification (TRAP) using an adipocyte-specific cre in adult wildtype mice followed by RNA-Seq Results: Using an optimized data analysis workflow, we mapped about 30 million sequence reads per sample to the mouse genome (build mm9) using Tophat and assembled reads into transcripts using Cufflinks. ribosomal affinity purified mRNA profiles of adult mice were generated by deep sequencing, in triplicate, using Illumina NextSeq500.
