Tumor progression is independent of tumor associated macrophages in two lineage-based mouse models of GBM [SortedTAM RNAseq]

We investigate whether TAM gene expression is similar between spontaneous and transplanted mouse models of GBM We sort Microglia and monocyte derived macrophages (MDM) from subventricular zone (type 1) and oligodendrocyte progenitor derived (type 2) spontaneous and transplanted mouse GBM tumors and perform bulk RNAseq Overall design: 3 Spontaneous GBM MDM, 6 spontaneous GBM microglia, 6 transplanted GBM MDM, 6 transplanted GBM microglia, 4 normal brain microglia (control)