Bacterial community in peanut vine silage

Ultimately, the quality of silage is highly contingent upon the microbial communities involved, their dynamic succession, and the resultant fermentation-derived metabolites. Therefore, understanding the interaction between bacteria and fungi during the silage process, particularly through the application of microbial sequencing technology, is of paramount importance. However, there is a paucity of studies addressing the changes in microbiological flora within peanut silage. Therefore, utilize high-throughput sequencing technology to analyze 16S ribosomal RNA and the internal transcribed spacer regions, thereby evaluating the effects of inoculants and substrates on the bacterial and fungal communities, as well as their interactions.