Single-cell RNA-seq of bone marrow cells from aplastic anemia patient and healthy donor

BM samples were collected from two adult healthy donors and two AA patients at the first hospital affiliated from Zhejiang Chinese University. Mononuclear cells (MNCs) were isolated using Ficoll-Hypaque gradient separation. CD34+ cells were purified from MNCs with the human anti-CD34 MicroBeads Isolation kit according to the manufactures specifications. Isolated cell was the purification of CD34+ cell. Then, MNCs mixed with CD34+ cells at 4:1 ratio and were analyzed by 10×Genomics. Overall design: a large-scale single-cell transcriptomic sequencing of 20,000 bone marrow cells from two adult healthy donors and two AA patients