Jennifer Bestman, Holly Cline (2011) CIL:37081, Xenopus laevis, CNS neuron (sensu Vertebrata). CIL. Dataset

This is IMAGE #1 (hour 0) of a developing neuron from the optic tectum of an albino Xenopus laevis tadpole CNS, stage 47.
The cell was imaged at a 2 hour intervals over 8 hours and shows the development and morphological plasticity of the dendritic arbor and axon over time.

The cell was transfected with in vivo electroporation of plasmid DNA encoding eGFP ~24 hours before the image was acquired. The intact, living tadpole was anesthetized, positioned under a glass coverslip and this image was taken directly through its transparent skin using a custom built two-photon microscope.

此为兔形蛙白化幼蟾蜍中枢神经系统（CNS）视顶盖中发育中的神经元图像 #1（0小时）。该细胞在2小时间隔下连续成像8小时，展现了树突丛和轴突随时间推移的发展及形态可塑性。细胞在成像前约24小时通过原位电穿孔转染编码绿色荧光蛋白（eGFP）的质粒DNA。实验中所用的完整、活着的幼蟾蜍经过麻醉处理，置于玻片下方，并通过定制的双光子显微镜直接穿透其透明皮肤进行成像。