The intestinal microbiota of channel catfish (Ictalurus punctatus) fed an iron sulphate-supplemented diet

Juvenile channel catfish (Ictalurus punctatus) were fed either a control or diet supplemented with iron sulphate (final concentration 1000 mg Fe/kg diet). Digesta was collected after a 63-day feeding trial for DNA extraction. Amplification of the V4 region of the 16S rRNA gene was performed, followed by sequencing on an Illumina MiSeq (2x300).