Knockout of PP1 in Leishmania major reveals a critical role for protein phosphatase 1 during RNA polymerase II transcription termination

Base J is a modified DNA base that is enriched at RNA polymerase II termination sites. We previously identified a complex in Leishmania major and T. brucei composed of PNUTS, PP1, Wdr82 and a J-binding protein (JBP3). We show that deletion of PP1 in L. major leads to readthrough transcription and expression of downstream genes. Overall design: The role of PP1in regulating transcription termination was investigated using inducible KO of PP1 in L. major. We used 6 RNA seq libraries to determine gene expression changes following the loss of PP1.