In-depth Profiling and Quantification of the Acetylproteome in Hepatocellular Carcinoma with a Trapped Ion Mobility Mass Spectrometer

In this study, we employed an integrated proteomics and acetyl-proteomics strategy to characterize of 8 tumors and 8 matched normal adjacent tissues (NATs) with 4D proteomics technology. Proteomics analysis identified a total of 6551 proteins, of which 5411 proteins were quantified across all samples. A total of 8789 acetylation sites on 2348 proteins were identified, of which 5112 sites of 1620 proteins contained quantitative information.