SNCA targeted gDNA: PacBio raw data

The landscape of SNCA transcripts across synucleinopathies: New insights from long reads sequencing analysis

gDNA capture using IDT xGen® Lockdown® Probes and single-molecule sequencing

2µg of each gDNA sample was sheared to 6kb using the Covaris g-TUBE and ligated with barcoded adapters. An equimolar pool of 12-plex barcoded gDNA library (2µg total) was input into the probe based capture with a custom designed SNCA gene panel.

A SMRTBell library was constructed using 626ng of captured and re-amplified gDNA (https://www.pacb.com/wp-content/uploads/Procedure-Checklist-%E2%80%93-Multiplex-Genomic-DNA-Target-Capture-Using-IDT-xGen-Lockdown-Probes.pdf).  A total of 3 SMRT Cells (6 hour movie) were sequenced on the PacBio Sequel platform using 2.0 chemistry.    

Barcodes used for gDNA demultiplexing

bc1001: gcagtcgaacatgtagctgactcaggtcacCACATATCAGAGTGCG
bc1002: gcagtcgaacatgtagctgactcaggtcacACACACAGACTGTGAG
bc1003: gcagtcgaacatgtagctgactcaggtcacACACATCTCGTGAGAG
bc1004: gcagtcgaacatgtagctgactcaggtcacCACGCACACACGCGCG
bc1005: gcagtcgaacatgtagctgactcaggtcacCACTCGACTCTCGCGT
bc1006: gcagtcgaacatgtagctgactcaggtcacCATATATATCAGCTGT
bc1007: gcagtcgaacatgtagctgactcaggtcacTCTGTATCTCTATGTG
bc1008: gcagtcgaacatgtagctgactcaggtcacACAGTCGAGCGCTGCG
bc1009: gcagtcgaacatgtagctgactcaggtcacACACACGCGAGACAGA
bc1010: gcagtcgaacatgtagctgactcaggtcacACGCGCTATCTCAGAG
bc1011: gcagtcgaacatgtagctgactcaggtcacCTATACGTATATCTAT
bc1012: gcagtcgaacatgtagctgactcaggtcacACACTAGATCGCGTGT