Transcriptomic profiling investigating altered cellular pathways upon QKI depletion in mouse eye lens tissue, mouse neural stem cell-derived lens progenitor-like cells (NLPCs), and human lens epithelial cell line (HLE-B3).

Significantly altered cellular pathways in the transcriptomic profiling upon QKI depletion potentially play an important role in protein homeostasis in eye lens cells. Overall design: Mouse eye lens tissues were isolated from the mice with tamoxifen-induced Qk deletion under the control of Nestin promoter and the control mice. NLPCs were differentiated from the neural stem cells isolated from Nestin-CreERT2; QkL/L, and 4OHT treatment induced the Qk deletion. HLE-B3 cells were obtained from ATCC (CRL-11421), and QKI KO was conducted using CRISPR-Cas9 system.