Cytokine secretion of hBMSCs.

hBMSC secretion profile of four cytokines and two growth factors was not influenced by SPIO labeling. Cytokines were measured in serum free 24 hour supernatant of labeled and unlabeled cells. SPIO did not induce the secretion of pro-inflammatory cytokines (TNFα, IFNγ), nor did it alter the secretion of the anti- and pro-inflammatory cytokine IL-6 or the growth factor VEGF. The anti-inflammatory factor IL-10 and growth factor FGF2 were not secreted at a detectable level by control or SPIO-labeled cells. TNF-α: tumor necrosis factor-alpha, IFN-γ: interferon-gamma, IL: interleukin, VEGF: vascular endothelial growth factor, FGF: fibroblast growth factor, ≤d.l.: below detection level (10 pg/ml). Results shown for duplicate samples from five hBMSC donors.