Output of immune status and serotype inferring models (Thao et al.,)

The serological data used in this study were processed by a novel multiplex protein microarray (PMA) developed. The PMA slides were printed at the Viroscience Department (Erasmus Medical Centre) as described elsewhere. The slides include several flavivirus antigens such as DENVs 1–4, Zika virus (ZIKV), West Nile virus (WNV), and St. Louis encephalitis virus (SLEV). Selected serum samples were tested for IgG antibodies against these viruses using the protocol previously described (25)⁠. Each serum sample was tested serially in 4-fold dilutions, from 1:20 to 1:1280. The antibody concentration indicator is the fluorescent signal with 3,000 and 65,535 as the lower and upper limits of detection respectively.