Distribution of viral genomic load† and influenza A antigen in tissues of stonechats after challenge infection with HPAIV H5N1.

†Viral RNA detected by real-time reverse transcription-PCR (rRT-PCR) in birds after challenge infection with highly pathogenic avian influenza virus strain A/Cygnus cygnus/Germany/R65/06 (H5N1). Real-time RT-PCR results are presented as cycle of threshold (Ct)-values: >35 scored as negative.*Statistical significant (alpha 0.05) difference between non-migrating and migrating populations according to association of unordered r x c tables by Fisher-Freeman-Halton's test.