A comparison of PATH and CDC real-time PCR data with the test panel scores from each developer.

The PATH and CDC datasets are shown as the mean Ct from three reactions per sample dilution. The developer datasets are depicted as the number of correct results scored for each sample (maximum 5) as compared to the PATH test data. Ct, mean cycle threshold; A–G denotes the different developers; F1, developer F used PCR amplification; F2, developer F used loop-mediated isothermal amplification; NT, not tested; N/A not applicable; CI, confidence interval.