PrbP modulates biofilm formation in Liberibacter crescens

RNA sequencing of L. crescens grown with tolfenamic acid and a DMSO vehicle control. Overall design: L. crescens BT-1 cells were grown with 10 µM tolfenamic acid (in 0.05% dimethyl sulfoxide) while the control was grown with 0.05% DMSO. L. crescens BT-1 cells were collected at OD600= 0.3 (mid-exponential phase) by centrifugation at 8000 rpm, at 4 °C. Total RNA was extracted with the RiboPure-Bacteria (Life Technologies) kit following the manufacturer's protocol. The quality of isolated RNA samples was examined in an Agilent 2100 Bioanalyzer. The rRNA was depleted using the MICROBExpress Bacterial mRNA enrichment kit (Life Technologies) according to the manufacturer's instructions. Single end RNA libraries were prepared using the TruSeq Stranded mRNA Library Prep Kit (Illumina) and followed by sequencing using a HiSeq2500 system. RNA sequencing was performed by the Genome Sciences Facility at Penn State University in Hershey, PA. The assays were performed in duplicates, and approximately 2million reads were obtained for each library.