Sequencing of reaction products from protospacer integration by Cas1-Cas2. Cloning vector pUC19
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https://www.ncbi.nlm.nih.gov/bioproject/PRJNA271290
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资源简介：
We report the sites of protospacer integration into plasmids by Cas1-Cas2. The goal of this study is to determine the role of sequence elements on protospacer integration. Overall design: Methods: Cas1-Cas2 integration products were fragmented, end-repaired, adapter ligated, PCR amplified, and analyzed by Illumina sequencing. Protospacer-containing reads were selected by Cutadapt and mapped to the plasmid with Bowtie.
创建时间：
2014-12-29
