Effects of benign and heat-stress conditions on parent clones and their selfed offspring of the pea aphid

In the face of global climate change, understanding the interaction between heat stress and inbreeding depression is a critical issue. We evaluated the magnitude of inbreeding depression under benign and heat-stress conditions using parent clones and their selfed offspring of Acyrthosiphon pisum. 
 
The number of nymphs produced by an adult during the first seven days was counted, and the total area of all nymphs was measured as fitness components of a clone. Inbreeding depression was observed during the development of the fundatrix and was exemplified by the occurrence of sterile fundatrices, which accounted for 28.6% of the adults. 
 
Once clonal lines were established, no significant overall differences were detected in these fitness components between a parent clone and its 30 selfed-offspring clones at a constant 20 °C. However, pairwise comparisons revealed a significant reduction in the fitness components in a few selfed clones, whereas one selfed clone signif..., In the first experiment, a single clone of the pea aphid (Acyrthosiphon pisum, clone 05E) and its selfed offspring clones were prepared. We evaluated the fecundity and growth rate of each clone at 20 °C under a 16L:8D photoperiod. From each clone, a single first-instar nymph was selected as the test aphid and transferred to a new seedling, where it was reared to adulthood. The first day of larviposition was recorded as day 1, and on day 7, all offspring produced were collected and preserved in 80% ethanol. For each of the 30 selfed clones, we prepared 10 to 12 replicates (mean = 11.4), and for the parental clone 05E, we prepared 13 replicates. The number of nymphs produced over the first seven days (7-day fecundity) was counted. All nymphs were placed in a petri dish containing a small amount of 80% ethanol, and their image was captured using a digital microscope (Dino-Lite Edge, AnMo Electronics) under standardized lighting conditions. Images were binarized using a consistent thre..., , # Effects of benign and heat-stress conditions on parent clones and their selfed offspring of the pea aphid

[https://doi.org/10.5061/dryad.ncjsxkt57](https://doi.org/10.5061/dryad.ncjsxkt57)

## Description of the data and file structure

Here, we provide three data files containing: (1) the number of nymphs produced by test aphids over the first seven days (7-day fecundity) and the total area occupied by the nymphs, (2) mortality data of aphids exposed to different temperature conditions, and (3) fecundity data of aphids exposed to different temperature conditions. The aphid clones used in the experiments are identified, and temperature treatments are coded as CT, SH, and RH. CT (control) indicates a constant temperature of 20 °C; SH (single heat stress) refers to 35 °C for 6 hours followed by 20 °C for the remaining time; and RH (repetitive heat stress) consists of 35 °C for 6 hours per day for three consecutive days, with 20 °C for the rest of the time.

### Files and variables

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