Whole exome sequencing of murine derived intestinal tumour organoids [exome-capture]. Mus musculus

DNA was isolated from Apcmin/+;KrasLSL-G12D/+;VillinCre;Lgr5DTReGFP (AKVL), Apcmin/+;KrasLSL-G12D/+;VillinCre;Lgr5DTReGFP;p53KO (AKVPL) and Apcmin/+;KrasLSL-G12D/+;VillinCre;Lgr5DTReGFP;p53KO,Smad4KO (AKVPSL) organoids as well as the spleen of the AKVL donor animal The "SAMPLE_ID" sample characteristic is a sample identifier internal to Genentech. Overall design: Whole exome sequencing of murine derived intestinal organoids containing increasing number of CRC driver mutations was performed to identify potential mutations in coding regions that were introduced by off-target binding of the CRISPR-Cas9 complex.