Lemon zinc finger protein ClSUP induces the accumulation of reactive oxygen species and inhibits citrus yellow vein-clearing virus infection via interactions with ClDOF3.4

Citrus yellow vein-clearing virus (CYVCV) is an increasing threat to citrus cultivation. Notably, the role of zinc finger proteins (ZFPs) in mediating viral resistance in citrus plants is unclear. In this study, we demonstrate that ZFPs ClSUP and ClDOF3.4 enhance citrus defense responses against CYVCV in Eureka lemon. ClSUP interacted with the coat protein (CP) of CYVCV to reduce CP accumulation and inhibit its silencing suppressor function. Overexpression of CISUP triggered reactive oxygen species (ROS) and salicylic acid (SA) pathways, and enhanced resistance to CYVCV infection. In contrast, ClSUP-silencing resulted in increased CP accumulation and down-regulated ROS and SA-related genes. ClDOF3.4 interacts with ClSUP to facilitate its interactions with CP. Furthermore, ClDOF3.4 synergistically regulated the accumulation of ROS and SA with ClSUP and accelerated the down-regulation of CP accumulation. Transgenic plants co-expressing ClSUP and ClDOF3.4 remarkedly decrease the CYVCV. The..., These data were obtained by RT-qPCR, ELISA assay, transcriptome sequencing analysis and other methods., , # Lemon zinc finger protein ClSUP induces the accumulation of reactive oxygen species and inhibits citrus yellow vein-clearing virus infection via interactions with ClDOF3.4

[https://doi.org/10.5061/dryad.jq2bvq8jp](https://doi.org/10.5061/dryad.jq2bvq8jp)

We have submitted our raw data of the figures (Data of figures.xls), raw data of the supporting figures (Data of supporting figures.xls), and raw data of the second reference genes (Data of the second reference genes.xls).

#### **Description of data**

**Data of figures.xls**

·Fig. 2D Relative expression level of *CP*: the relative expression level of *CP* at GFP+CP+GUS-His and GFP+CP+ClSUP-His infiltrated patches, GFP+CP+GUS-His was used as control; *Actin* was utilized as an internal control, analyzed by the 2−ΔΔCt method.

·Fig. 2G Relative expression level of *ClSUP*: the relative expression level of *ClSUP* in pGBi:GFP and pGBi:ClSUP-GFP infiltrated Eureka lemon leaves at 0, 4, 8, 12, and 16 dpi, the data of 0 dpi pGBi:GFP wa...