RNA seq of mouse CD3 T cells

Purpose: To gain more mechanistic insights into the effects of SDHB deletion in T cells, we performed RNAseq in activated WT and SDHB cKO T cells. Methods: Total RNA was isolated according to the RNeasy Mini Kit (Qiagen) and treated with DNase I. RNA quality was assessed using the Agilent 2100 Bioanalyzer and RNA Nano Chip kit (Agilent Technologies, CA). Results: RNA-seq revealed an enriched pro-inflammatory gene signature in SDHB cKO T cells after activation Conclusions: SDH inactivation results in a pro-inflammatory gene signature in T cells after activation Overall design: mRNA profiles of activated WT and SDHB KO T cells were generated by deep sequencing, using Illumina GAIIx.