Metagenomic analysis in mice subjected to UCMS stress and treated with L. casei IDCC 3451.

To investigate the effect of L. casei IDCC 3451 on behavioral impairments in mice induced by UCMS (unpredictable chronic mild stress), the mice were categorized into four groups: CONT (control group), UCMS (mice exposed to unpredictable chronic mild stress), 3451 (UCMS mice treated with L. casei IDCC 3451), and LGG (UCMS mice treated with L. rhamnosus GG as a positive control). Each group consisted of 15 biosamples, with fecal samples collected and analyzed by group. Genomic analysis of the fecal microbiota for each group was performed, with the study including 8 replicates of each analysis.