Stroma extracts were isolated from 2-week-old WT plants and incubated with either PUMPKIN-specific antibodies or with the pre-immune serum. IgGs were captured with SiMAG-Protein G beads (Chemicell) an
To identify potential miRNA–mediated targets associated with osteopetrosis at the genome-wide level, two small RNA libraries and a degradome library were constructed from the ADO2-iPSCs and normal con
High-throughput sequencing of RNAs isolated by cross-linking immunoprecipitation in Metarhizium robertsii Overall design: we isolated and identified small RNAs including miRNAs and their targets assoc
Total poly(A) RNAs from wild-type adult stage worms were subjected to high-throughput sequencing using an Illumina platform to detect RNA cleavage fragment. Overall design: Wild-type (N2) C. elegans