Epigenomes of clonal palms with different fruit phenotypes.

Leaf tissue was sampled from normal and mantled clonal palms producing normal and mantled fruits respectively. ChIP-Seq libraries for histone modifications H3K4me3 and H3K27me3 were generated and sequenced on Illumina platform. Genomic regions associated with specific histone marks were identified. Overall design: Leaf tissue used in this study was sampled from clonal palms derived from a single ortet (mother palm). These 10 clonal palms comprised 5 normal and 5 mantled phenotype palms. Leaf tissues of frond 0 were sampled and used for isolation of chromatin. Chromatin was sheared and a portion of sheared chromatin was set aside for ChIP assay. Sheared chromatin was then immunoprecipitated with H3K4me3- and H3K27me3-mouse monoclonal antibodies (ChIP grade). ChIP-Seq libraries were prepared using DNA from non-immunoprecipitated and ChIP assay. Paired-end sequencing was conducted. Sequencing reads were analysed to identify regions in oil palm genome associated with H3K4me3 and H3K27me3 histone marks.