Megalobrama amblycephala Raw sequence reads. Megalobrama amblycephala

Using Illumina RNA-seq technology, liver sequence reads for Megalobrama amblycephala were generated under control and handling stress conditions. We identified 785 up-regulated and 167 down-regulated transcripts. Gene ontology enrichment analysis revealed a significant upregulation of catabolic genes associated with skeletal muscle atrophy. These results were validated by RT-qPCR analysis for ten candidates genes involved in oxidative stress in liver. Additionally, using a primary culture of fish liver cells, the effect of cortisol was evaluated in relation to liver damage.